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ROS also interact with unsaturated fatty acids in phospholipids, and aldehydes such as 4-hydroxynonenal, 4-hydroxyhexanal, acrolein, and MDA are the most reactive compounds (87)

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APEX2 (2 µM) was incubated with Amplex Red (100 µM), GSH (1 mM), plus/minus Na 2 S 4 (10 µM), for 15 min, while recording resorufin formation (left panel)