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Reverse transcription and Real Time PCR were performed using PrimeScript™ RT Reagent Kit with gDNA Eraser and TB Green ® Premix Ex Taq™ II (Takara Biotech Co., Ltd., Dalian, China) at Bio-Rad CFX Connect fluorescent PCR amplifier (Bio-Rad, Hercules, CA, USA) according to the supplier’s instructions

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