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In another study, which was conducted on the effect of vitamin B12 deficiency on colon tissue damage caused by DSS due to changes in intestinal microbiota, it was found that supplementing with this vitamin at high dose (200 μg/kg/d for 4 weeks) can improve intestinal microbiota and have a positive effect on colitis tissue lesions.[38] Therefore, another possibility to explain the effectiveness of group B vitamins in colitis, especially in high doses, could be their ability to improve and modify the intestinal microbiota, which needs further investigation

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Since many pathologic and toxic conditions result in sustained increase in cytosolic free Ca 2+ , which can lead to activation of CMK and phosphorylation of GCLC, theoretically, inhibition of GCL may further contribute to toxicity under these conditions