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They tend to fall into two categories

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The RNA was dissolved in 20 µl of dH 2 O and 3 µg was used in a 20 µl reaction volume for a first strand cDNA synthesis using SuperScript™ II RT (Invitrogen by Fisher Scientific, USA) according to the instructions using oligo dT priming with oligo (dT) 12-18 primer (Invitrogen by Life Technologies, USA)