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For histology and immunohistochemical detection, the organoids were briefly rinsed in 0.1 M Sörensen buffer (pH 7.4) and immersed in 3% PFA and 0.05% glutaraldehyde in 0.1 M Sörensen buffer (pH 7.4) for 2 hours at room temperature followed by 30 minutes at 4 °C