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Moreover, the cell proliferation ability of HCC cell lines (MHCC97H and HepG2) and PCa cell lines (LNCaP and C4-2) increased with an increase in Gln concentration, as determined by cell CCK- 8 and colony formation assays (Fig

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Reduced roGFP2 was obtained after a 20 min treatment with 10 mM DTT, which was subsequently removed on a ZebaTM Spin Desalting Column (Thermo Scientific)