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This rapid increase in absorbance is assumed to be due to the leakage of low molecular weight compounds absorbing at 260 nm (ATP, nucleosides, and nucleotides) and at 280 nm (amino acids and small peptides that are free in the cytoplasm)

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Determination of Michaelis-Menten constant for glutathione In these experiments, the concentration of monobromobimane was kept constant at 50 μM, and concentration of glutathione was varied