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2.8.2 Data analysis of solute carrier (SLC) substrate and inhibition study Regarding the substrate assay, the concentrations of the compounds or probe substrates in the cell lysate were determined using LC-MS and used to calculate the uptake rate (pmol/mg/min) using Equation 3.where, C lysate , P, and T represent the concentrations detected in the cell lysate (pmol), protein concentration in the cells (mg), and incubation time (min), respectively